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KMID : 1161420160190040398
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Journal of Medicinal Food
2016 Volume.19 No. 4 p.398 ~ p.403
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The Antiproliferative Effect of Moringa oleifera Crude Aqueous Leaf Extract on Human Esophageal Cancer Cells
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Tiloke Charlette
Phulukdaree Alisa
Chuturgoon Anil A.
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Abstract
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Esophageal cancer (EC) is commonly diagnosed in South Africa (SA), with high incidences occurring in SA's black population. Moringa oleifera (MO), a multipurpose tree, is used traditionally for its nutritional and medicinal properties. It has been used for the treatment of a variety of ailments, including cancer. We investigated the antiproliferative effect of MO crude aqueous leaf extract (MOE) on a cancerous esophageal cell line (SNO). SNO cells were exposed to a range of MOE dilutions to evaluate cytotoxicity (MTT assay). Oxidative stress was determined using the TBARS assay. The comet assay was used to assess DNA damage. We then determined cell death mechanisms by measuring phosphatidylserine (PS) externalization (flow cytometry), caspase-3/7 and caspase-9 activities, and adenosine triphosphate (ATP) levels (luminometry). Protein expression of Smac/DIABLO and PARP-1 was determined by western blotting. SNO cells were treated with a range of MOE dilutions to obtain an IC50 value of 389.2?μg/mL MOE (24?h), which was used in all subsequent assays. MOE significantly increased lipid peroxidation (P?
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KEYWORD
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apoptosis, DNA fragmentation, oxidative stress, SNO cancer cells
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